Distinguished Professor
Chen, Shu-Hui
Analytical Chemistry, Mass Spectrometry
Separation Science, Proteomics
Biochemical Analysis
✉ Email：shchen@mail.ncku.edu.tw ☎ Phone：06-2757575-65339
Education
BS in Chemistry, National Tsing Hua University (1983)
MS in Chemistry, National Taiwan University (1986)
PhD in Chemistry, Michigan State University (1993)
Experience
1983-1984 National Tsing Hua University, Assistant
1996-2000 National Cheng Kung University, Associate Professor
2000-Present National Cheng Kung University, Professor
Publications
1.
Kafeenah, H.; Kuo, C.M.; Chang, T.Y.; Jen, H.H.; Yang, J.H.; Shen, YS.; Wu, C.H.; Chen, S.H.*"Label-free and de-conjugation-free workflow to simultaneously quantify trace amount of free /conjugated and protein-bound estrogen metabolites in human serum" Analytica Chimica Acta, 2022, 1232, 340457.
2.
Akbarian, M.*; Bahmani, M.; Chen, S.H.; Yousefi, R.; Mohammadi-Samani, S.; Tayebi, L.; Panahi, F.; Farjadian, F.*"Mechanisms behind the Fibrillation and Toxicity of Insulin Fibrils on Neuron Cells by Engineered Curcumin Analogs" ACS Chem. Neurosci., 2022, 13, 17, 2613–2631.
3.
Huang, W.C.; Huang, L.J.; Hsu, L.S.; Huang, S.T.; Lo, W.T.; Wang, T.F.; Sun, W.T.; Wei, W.Y.; Lee, Y.S.; Chuang, S.H.; Lee, C.P.; Chou, H.H.*; Chen, S.H.* "Selective and predicable amine conjugation sites by kinetic characterization under excess reagents" Scientific Reports, 2021, 11, 21222.
4.
Jen, H.H. ; Kafeenah, H.; Chang, T.Y. ; Lin, Y.M. ; Shan, Y.S. ; Wu, C.H.; Chen, S. H.* "Quantification of the Endogenous Adduction Level on Hemoglobin and Correlation with Albumin Adduction via Proteomics: Multiple Exposure Markers of Catechol Estrogen" J. Proteome Res., 2021, 20, 9, 4248–4257.
6.
Kuo, C.M.;Wei, S.Y. ;Du, S.H. ;Lin, J.L. ;Chu, C.H. ;Chen, C.H.;Tai, J.H. ; Chen, S. H. * "Comprehensive Workflow for Mapping Disulfide Linkages Including Free Thiols and Error Checking by On-Line UV-Induced Precolumn Reduction and Spiked Control" Anal. Chem., 2021, 93, 3, 1544-1552.
7.
Do, Q.T. ; Huang, T.E. ; Liu, Y.C. ; Tai, J.H.; Chen, S. H. * "Identification of Cytosolic Protein Targets of Catechol Estrogens in Breast Cancer Cells Using a Click Chemistry-Based Workflow" J. Proteome Res., 2021, 20, 1, 624-633.
8.
Hsu, H. M.; Huang, Y. H.; Sarita Aryal; Liu, H. W.; Chinpan Chen; Chen, S. H.; Chu, C. H.*; Tai, J. H.* "Endomembrane Protein Trafficking Regulated by a TvCyP2 cyclophilin in the protozoan parasite, Trichomonas vaginalis" Sci. Rep., 2020, 10, 1275.
9.
Lin, H. S.; Yan Wu; Liu, Y. J.; Chen, S. H.*; Chen, W. T.*; Wang, S. P.* "Comments on chemical properties reported for diphenyl disulfide and its derivatives: The merit of the phenyl groups" J. Chin. Chem. Soc., 2019, 67, 324–328.
10.
Huang, Y. S.; Lin, Y. M.; Chen, Hsin; Wu, C. H.; Syu, C. H.; Huang, T. E.; Do, Q. T.; Chen, S. Y.* "Targeting Endogenous Adduction Level of Serum Albumin by Parallel Reaction Monitoring via Standard Additions and Intact Protein Measurement: Biological Dosimetry of Catechol Estrogens" Anal. Chem., 2019, 91, 15922-15931.
11.
Hsu, Y. C.; Hung, M. J.; Chen, Y. A.; Wang, T. F.; Ou, Y. R.; Chen, S. H.* "Identifying Reducing and Capping Sites of Protein-Encapsulated Gold Nanoclusters" Molecules, 2019, 24, 1630.
12.
Huang, L. J.; Chiang, C. W.; Chen, S. L.; Wei,S. Y.; Chen, S. H.* "Complete mapping of disulfide linkages for etanercept products by multi-enzyme digestion coupled with LC-MS/MS using multi-fragmentations including CID and ETD" J. Food Drug Anal., 2019, 27, 531-541.
13.
Huei-Chen Liang; Yi-Chen Liu; Hsin Chen; Ming-Chun Ku; Quynh-Trang Do; Chih-Yen Wang;
Shun-Fen Tzeng; Chen, S. H.* "In Situ Click Reaction Coupled with Quantitative Proteomics
for Identifying Protein Targets of Catechol Estrogens" J. Proteome Res., 2018, 17, 2590-2599.
14.
Hung Wen Tsai; Chung Liang Ho; Shu Wen Cheng; Yih Jyh Lin; Chou Cheng Chen; Pin Nan Cheng; Chia Jui Yen; Ting Tsung Chang; Po Min Chiang; Shih Huang Chan; Cheng Hsun Ho; Chen, S. H.; Yi Wen Wang; Nan Haw Chow; Jou Chun Lin "Progesterone receptor membrane component 1 as a potential prognostic biomarker for hepatocellular carcinoma" World J Gastroenterol., 2018, 24, 1152-1166.
15.
Jhan, S. Y.; Huang, L. J.; Wang, T. F.; Chou, H. H; Chen, S. H.* "Dimethyl Labeling Coupled with Mass Spectrometry for Topographical Characterization of Primary Amines on Monoclonal Antibodies" Anal. Chem., 2017, 89, 4255-4263.
16.
Ho, C. H.; Tsai, H. W.; Lee, C. Y.; Huang, L. J.; Chien, R. N.; Wu, I. C.; Chiu, Y. C.; Liu, W. C.; Cheng, P. N.; Chang, T. T.; Chen, S. H.* "Favorable Response to Long-term Nucleos(t)ide Analogue Therapy in HBeAg-positive Patients with High Serum Fucosyl-Agalactosyl IgG" Sci. Rep., 2017, 7, 1957.
17.
Huang, L. J.; Chiang, C. W.; Lee, Y. W.; Wang, T. F.; Fong, C. C.; Chen, S. H.* "Characterization and comparability of stress-induced oxidation and deamidation on vulnerable sites of etanercept products" J. Chromatogr. B, 2016, 1032, 189-197.
18.
Ku, M. C.; Fang, C. M.; Cheng, J. T.; Liang, H. C.; Wang, T. F.; Wu, C. H.; Chen, C. C.; Tai, J. H.; Chen, S. H.* "Site-specific covalent modifications of human insulin by catechol estrogens: Reactivity and induced structural and functional change" Sci. Rep., 2016, 6, 28804.
19.
Tsai, C. Y.; Li, C. W.*; Li, J. R.; Jang, B. H.; Chen, S. H.* "Steroid Probes Conjugated with Protein-Protected Gold Nanocluster: Specific and Rapid Fluorescence Imaging of Steroid Receptors in Target Cells" J. Fluoresc., 2016, 26, 1239-1248.
20.
Hong Yi Chang; Ting Chia Chang; Wen-Ya Huang; Chung-Ta Lee; Chia-Jui Yen; Yuh Shyan Tsai; Tzong Shin Tzai; Chen, S. H.; Nan-Haw Chow* "RON nuclear translocation under hypoxia potentiates chemoresistance to DNA double-strand break-inducing anticancer drugs" Mol. Canc. Therapeut., 2016, 15, 276-286.